Journal: PLoS ONE

Article Title: Circular RNA expression profiling of human granulosa cells during maternal aging reveals novel transcripts associated with assisted reproductive technology outcomes

doi: 10.1371/journal.pone.0177888

Figure Lengend Snippet: CircRNA_103827 and circRNA_104816 expressions in granulosa cells according to patients’ clinical characteristics.

Article Snippet: In stage 1, GCs samples from women of advanced age (AA, ≥ 38 years, n = 3) and young age (YA, ≤ 30 years, n = 3) were analyzed by a human circRNA microarray (8×15K, Arraystar Inc.).

Techniques: Biomarker Discovery

Journal: PLoS ONE

Article Title: Circular RNA expression profiling of human granulosa cells during maternal aging reveals novel transcripts associated with assisted reproductive technology outcomes

doi: 10.1371/journal.pone.0177888

Figure Lengend Snippet: CircRNA_103827 and circRNA_104816 expression levels in granulosa cells according to ART outcomes.

Article Snippet: In stage 1, GCs samples from women of advanced age (AA, ≥ 38 years, n = 3) and young age (YA, ≤ 30 years, n = 3) were analyzed by a human circRNA microarray (8×15K, Arraystar Inc.).

Techniques: Expressing

Journal: PLoS ONE

Article Title: Circular RNA expression profiling of human granulosa cells during maternal aging reveals novel transcripts associated with assisted reproductive technology outcomes

doi: 10.1371/journal.pone.0177888

Figure Lengend Snippet: The top 10 significantly differentially expressed circRNAs between young and older women ranked by fold change.

Article Snippet: In stage 1, GCs samples from women of advanced age (AA, ≥ 38 years, n = 3) and young age (YA, ≤ 30 years, n = 3) were analyzed by a human circRNA microarray (8×15K, Arraystar Inc.).

Techniques:

Journal: PLoS ONE

Article Title: Circular RNA expression profiling of human granulosa cells during maternal aging reveals novel transcripts associated with assisted reproductive technology outcomes

doi: 10.1371/journal.pone.0177888

Figure Lengend Snippet: Comparison of candidate circRNAs (circRNA_103829, circRNA_10827, circRNA_104816, circRNA_101889, circRNA_103828, circRNA_100833, circRNA_104852, circRNA_103611) expression levels in granulosa cells from additional young (n = 20) and older (n = 20) women by qRT-PCR. YA, women with young age (≤ 30 years); AA, women with advanced age (≥ 38 years). P values were calculated by Mann-Whitney U test. Relative expressions were analyzed by 2 -ΔΔCt method which was normalized to GAPDH. For each box plotting, the central mark represents the median, the edges of the box represent the 25 th and 75 th percentiles, and the whiskers are the most extreme data points not considered outliers.

Article Snippet: In stage 1, GCs samples from women of advanced age (AA, ≥ 38 years, n = 3) and young age (YA, ≤ 30 years, n = 3) were analyzed by a human circRNA microarray (8×15K, Arraystar Inc.).

Techniques: Comparison, Expressing, Quantitative RT-PCR, MANN-WHITNEY

Journal: PLoS ONE

Article Title: Circular RNA expression profiling of human granulosa cells during maternal aging reveals novel transcripts associated with assisted reproductive technology outcomes

doi: 10.1371/journal.pone.0177888

Figure Lengend Snippet: ( A-E ) circRNA_103827 expression levels in granulosa cells according to (A) serum AMH levels, (B) AFC, (C) retrieved oocytes, (D) top quality embryos and (E) top quality embryo percentage. ( F-J ) circRNA_104816 expression levels in granulosa cells according to (F) serum AMH levels, (G) AFC, (H) retrieved oocytes, (I) top quality embryos and (J) top quality embryo percentage.

Article Snippet: In stage 1, GCs samples from women of advanced age (AA, ≥ 38 years, n = 3) and young age (YA, ≤ 30 years, n = 3) were analyzed by a human circRNA microarray (8×15K, Arraystar Inc.).

Techniques: Expressing

Journal: PLoS ONE

Article Title: Circular RNA expression profiling of human granulosa cells during maternal aging reveals novel transcripts associated with assisted reproductive technology outcomes

doi: 10.1371/journal.pone.0177888

Figure Lengend Snippet: ROC analysis to evaluate predictive performance of circRNA_103827, circRNA_104816 expressions in granulosa cells and top quality embryo proportion for ( A-C ) clinical pregnancy outcomes, as well as for ( D-F ) live births. AUC, area under the ROC curve.

Article Snippet: In stage 1, GCs samples from women of advanced age (AA, ≥ 38 years, n = 3) and young age (YA, ≤ 30 years, n = 3) were analyzed by a human circRNA microarray (8×15K, Arraystar Inc.).

Techniques:

Journal: PLoS ONE

Article Title: Circular RNA expression profiling of human granulosa cells during maternal aging reveals novel transcripts associated with assisted reproductive technology outcomes

doi: 10.1371/journal.pone.0177888

Figure Lengend Snippet: (A) CircRNA_103827/circRNA_104816 targeted “Top 5” miRNA-gene network was portrayed based on sequence-pairing prediction. A pink round node represents a gene, a blue square represents miRNA, and a yellow diamond represents a circRNA. Overlapping genes of both circRNAs in this interactive network were ANKRD20A9P , XIST and KCNQ1OT1 . (B) GO and (C) KEGG pathway analysis of circRNA_103827 and circRNA_104816 predicted target genes. The top 10 significantly enriched activities and their scores (negative logarithm of P value) were listed in the X-axis and the Y-axis, respectively.

Article Snippet: In stage 1, GCs samples from women of advanced age (AA, ≥ 38 years, n = 3) and young age (YA, ≤ 30 years, n = 3) were analyzed by a human circRNA microarray (8×15K, Arraystar Inc.).

Techniques: Sequencing

Journal: Stroke

Article Title: Circular RNA expression Profiles Alter Significantly in Mouse Brain after Transient Focal Ischemia

doi: 10.1161/STROKEAHA.117.017469

Figure Lengend Snippet: Majority (~80%) of the circRNAs altered after stroke are exonic (A). The circRNAs altered after stroke were transcribed from all 20 chromosomes (B). Real-time PCR confirmed the microarray data for 6 circRNAs (3 upregulated and 3 downregulated) at 6h reperfusion compared to sham (C). Values are mean ± SD (n =3/group). Real-time PCR analysis was conducted in triplicate. *p<0.05 compared to sham (Student’s T-test).

Article Snippet: The fluorophore-labeled cRNAs were purified (RNeasy Mini Kit; Qiagen USA) and 1 μg sample of each labeled cRNA was fragmented (60°C for 30 min in fragmentation buffer/blocking buffer; Arraystar USA), suspended in hybridization buffer and hybridized to a mouse circRNA microarray (8×15K, Arraystar USA).

Techniques: Real-time Polymerase Chain Reaction, Microarray

Journal: Cancer Management and Research

Article Title: Profiles of differentially expressed circRNAs in esophageal and breast cancer

doi: 10.2147/CMAR.S167863

Figure Lengend Snippet: General information of ESCC patients for microarray test

Article Snippet: The RNA was isolated with an RNeasy mini kit (Qiagen, Hilden, Germany) and analyzed using an 8*15K Arraystar human circRNA microarray V2 (Catalog No: AS-CR-H-V2.0).

Techniques: Microarray

Journal: Cancer Management and Research

Article Title: Profiles of differentially expressed circRNAs in esophageal and breast cancer

doi: 10.2147/CMAR.S167863

Figure Lengend Snippet: General information of BC patients for microarray test

Article Snippet: The RNA was isolated with an RNeasy mini kit (Qiagen, Hilden, Germany) and analyzed using an 8*15K Arraystar human circRNA microarray V2 (Catalog No: AS-CR-H-V2.0).

Techniques: Microarray

Journal: Biological Research

Article Title: Microarray profiling and functional analysis of differentially expressed plasma exosomal circular RNAs in Graves’ disease

doi: 10.1186/s40659-020-00299-y

Figure Lengend Snippet: The list of differential expressed circRNAs in plasma exosome samples from patients with GD and healthy control subjects

Article Snippet: The circRNA expression microarray slide (AS-S-CR-H-V2.0, Arraystar Human circRNA Arrays V2, 8 × 15 K; Arraystar Inc.) was used.

Techniques: Clinical Proteomics, Control

Journal: Biological Research

Article Title: Microarray profiling and functional analysis of differentially expressed plasma exosomal circular RNAs in Graves’ disease

doi: 10.1186/s40659-020-00299-y

Figure Lengend Snippet: The circRNA-miRNA-mRNA regulatory network of hsa_circRNA_000102. CircRNA, miRNA, and mRNA are indicated as spheres in brown, red, blue color, respectively. CircRNA circular RNA, miRNA microRNA, mRNA messenger RNA

Article Snippet: The circRNA expression microarray slide (AS-S-CR-H-V2.0, Arraystar Human circRNA Arrays V2, 8 × 15 K; Arraystar Inc.) was used.

Techniques:

Journal: Biological Research

Article Title: Microarray profiling and functional analysis of differentially expressed plasma exosomal circular RNAs in Graves’ disease

doi: 10.1186/s40659-020-00299-y

Figure Lengend Snippet: The top 10 significantly enriched terms of biological process by GO analysis of hsa_circRNA_000102 associated genes. GO analysis was divided into three parts: biological process, cell component and molecular function

Article Snippet: The circRNA expression microarray slide (AS-S-CR-H-V2.0, Arraystar Human circRNA Arrays V2, 8 × 15 K; Arraystar Inc.) was used.

Techniques:

Journal: Biological Research

Article Title: Microarray profiling and functional analysis of differentially expressed plasma exosomal circular RNAs in Graves’ disease

doi: 10.1186/s40659-020-00299-y

Figure Lengend Snippet: The top 10 significant enriched pathways by KEGG pathway analysis of hsa_circRNA_000102 associated genes

Article Snippet: The circRNA expression microarray slide (AS-S-CR-H-V2.0, Arraystar Human circRNA Arrays V2, 8 × 15 K; Arraystar Inc.) was used.

Techniques:

Journal: Advances in drug and alcohol research

Article Title: Hippocampal ceRNA networks from chronic intermittent ethanol vapor-exposed male mice and functional analysis of top-ranked lncRNA genes for ethanol drinking phenotypes

doi: 10.3389/adar.2022.10831

Figure Lengend Snippet: Schematic diagram detailing the experimental pipeline utilized to generate the list of top novel ethanol-responsive hub lncRNA candidates to target for ethanol-related functional interrogation. Male mice were given a priming injection of either ethanol and pyrazole or saline and pyrazole and placed in either an ethanol- or room-air vapor champers for 16 hours/day, 4 days/week, for 7 weeks, respectively. Hippocampi were dissected 24 hours after the final vapor exposure and then subject to mRNA, lncRNA, circRNA, and miRNA microarray analysis. These data sets were then used to generate ceRNA networks of ethanol-responsive RNA genes.

Article Snippet: For circRNA analysis, Arraystar Inc. isolated total RNA, digested with RNase R (Epicentre, Inc.), fluorescently labeled (Arraystar Super RNA Labeling Kit), and subsequently hybridized to Arraystar Mouse circRNA Array V2 (8×15K).

Techniques: Functional Assay, Injection, Saline, Microarray

Journal: Advances in drug and alcohol research

Article Title: Hippocampal ceRNA networks from chronic intermittent ethanol vapor-exposed male mice and functional analysis of top-ranked lncRNA genes for ethanol drinking phenotypes

doi: 10.3389/adar.2022.10831

Figure Lengend Snippet: Volcano plots showing differential RNA expression based on log2 fold-change in expression (x-axis) and log10 p-value (y-axis) for (A) protein-coding RNA (mRNA), (B) long non-coding RNA (lncRNA), (C) circular RNA (circRNA), and (D) microRNA (miRNA). Each point indicates an individual non-duplicated probe on the microarray with blue = significantly down-regulated, red = significantly up-regulated, and black = non-significant. Significance is defined by p < 0.05.

Article Snippet: For circRNA analysis, Arraystar Inc. isolated total RNA, digested with RNase R (Epicentre, Inc.), fluorescently labeled (Arraystar Super RNA Labeling Kit), and subsequently hybridized to Arraystar Mouse circRNA Array V2 (8×15K).

Techniques: RNA Expression, Expressing, Microarray